Journal: Regenerative Biomaterials

Article Title: A sustained release of BMP2 in urine-derived stem cells enhances the osteogenic differentiation and the potential of bone regeneration

doi: 10.1093/rb/rbac015

Figure Lengend Snippet: Osteogenic differentiation in 2D co-culture. ( A ) ARS staining and quantification at Days 7, 14 and 21. Scale bar = 100 μm. ( B ) ALP staining and quantification at Days 4, 7, 14 and 21. Scale bar = 100 μm. * P < 0.05, ** P < 0.01

Article Snippet: Calcium deposits were visualized using ARS staining (Cyagen, China) at Days 7, 14 and 21.

Techniques: Co-Culture Assay, Staining

Journal: BioMed Research International

Article Title: miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

doi: 10.1155/2020/6139469

Figure Lengend Snippet: miR-187-5p was upregulated during osteogenic differentiation of BMSCs. (a) ALP staining was performed on day 14 after osteoblastic differentiation. Scale bar, 100 μ m. (b) ARS staining was performed on day 14 after osteoblastic differentiation. Scale bar, 100 μ m. (c) qRT-PCR analysis of the levels of miR-187-5p in BMSCs for 0 days, 7 days, and 14 days after osteogenic medium induction. (d) qRT-PCR was applied to detect transfection efficiency after transfection of miR-187-5p mimics, mimics-NC, miR-187-5p inhibitor, and inhibitor-NC for 24 h in BMSC cells. The data are presented as mean ± SEM of 3 independent experiments ( n = 3). ∗ p < 0.05 and ∗∗∗ p < 0.001.

Article Snippet: Then, cells were rinsed with distilled water three times and stained with 2% ARS staining solution (Cyagen Biosciences, USA) for 15 minutes at RT.

Techniques: Staining, Quantitative RT-PCR, Transfection

Journal: BioMed Research International

Article Title: miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

doi: 10.1155/2020/6139469

Figure Lengend Snippet: The overexpression of microRNA-187-5p promoted the osteogenic differentiation of BMSCs. (a) ALP staining was applied to detect the osteogenic differentiation of BMSCs. Scale bar, 100 μ m. (b) ARS staining was used to determine the osteogenic differentiation of BMSCs. Scale bar, 100 μ m. (c–e) The protein levels of OSX, OPN, and Runx2 in response to miR-187-5p expression, determined by western blot assays at 72 h after transfection. The data are presented as mean ± SEM of 3 independent experiments ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Then, cells were rinsed with distilled water three times and stained with 2% ARS staining solution (Cyagen Biosciences, USA) for 15 minutes at RT.

Techniques: Over Expression, Staining, Expressing, Western Blot, Transfection

Journal: BioMed Research International

Article Title: miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

doi: 10.1155/2020/6139469

Figure Lengend Snippet: The knockdown of microRNA-187-5p inhibited the osteogenic differentiation of BMSCs. (a) ALP staining was used to determine the osteogenic differentiation of BMSCs. Scale bar, 100 μ m. (b) ARS staining was used to determine the osteogenic differentiation of BMSCs. Scale bar, 100 μ m. (c, d) The protein levels of OSX, OPN, and Runx2 in response to miR-187-5p inhibition, determined by western blot assays at 72 h after inhibition or NC transfection. The data are presented as mean ± SEM of 3 independent experiments. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Then, cells were rinsed with distilled water three times and stained with 2% ARS staining solution (Cyagen Biosciences, USA) for 15 minutes at RT.

Techniques: Knockdown, Staining, Inhibition, Western Blot, Transfection

Journal: BioMed Research International

Article Title: miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

doi: 10.1155/2020/6139469

Figure Lengend Snippet: ICAM-1 silence promoted the osteogenic differentiation of BMSCs. (a) ALP staining was applied to detect the osteogenic differentiation of BMSCs. Scale bar, 100 μ m. (b) ARS staining was used to determine the osteogenic differentiation of BMSCs. Scale bar, 100 μ m. (c–e) The protein levels of OSX, OPN, and Runx2 in response to ICAM-1 silence, determined by western blot assays at 72 h after transfection. The data are presented as mean ± SEM of 3 independent experiments ( n = 3). ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: Then, cells were rinsed with distilled water three times and stained with 2% ARS staining solution (Cyagen Biosciences, USA) for 15 minutes at RT.

Techniques: Staining, Western Blot, Transfection

Journal: RSC Advances

Article Title: Polydopamine-functionalized acellular fish scale scaffolds for accelerated bone tissue regeneration †

doi: 10.1039/d5ra01932j

Figure Lengend Snippet: Osteogenic differentiation ability of scaffolds in vitro . (A) ALP staining and ALP activity of BMSCs after 7 and 14 days of osteogenic induction, and ARS staining and quantitative analysis of calcium nodules after 21 days of osteogenic induction. Scale bar = 200 μm. (B and C) Fluorescence microscopy images and quantitative analysis of IF staining of RUNX2, OPN, and COL-1α in BMSCs after 14 days of osteogenic induction. Scale bar = 100 μm. (D) The mRNA expression levels of RUNX2, OPN, and COL-1α in BMSCs after 14 days of osteogenic induction. All values are presented as means ± standard deviation (ns: no significant differences, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001).

Article Snippet: After 21 days of osteogenic induction, cells were fixed with 4% paraformaldehyde and stained using an ARS kit (Solarbio, China) ( n = 3).

Techniques: In Vitro, Staining, Activity Assay, Fluorescence, Microscopy, Expressing, Standard Deviation